Elements of Biotechnology Lab (1BEBTL107/207)

Elements of Biotechnology Lab

Course Code 1BEBTL107/207 
Semester I/II
CIE Marks 50
Teaching Hours/Week (L:T:P:S) 0:0:2:0 
SEE Marks 50
Total Hours of Pedagogy 24 
Total Marks 100
Credits 1 
Exam Hours 3
Examination type (SEE) Practical

PART – A

COVENTIONAL EXPERIMENTS

Biochemical Estimations

1. Preparation of standard buffers

2. Estimation of carbohydrates and protein with error analysis

Microbial techniques

3. Sterilization of glassware using dry and wet heat

Microscopy & Staining

4. Onion root tip — stages of mitosis & mitotic index

5. Cell viability studies with Trypan Blue

6. Observation of prokaryotic and eukaryotic cells (Preparation of permanent slides)

PART – B

TYPICAL OPEN-ENDED EXPERIMENTS

Open-ended experiments are a type of laboratory activity where the outcome is not predetermined, and

students are given the freedom to explore, design, and conduct the experiment based on the problem

statements as per the concepts defined by the course coordinator. It encourages creativity, critical thinking, and

inquiry-based learning.

1. Concept 1: Antimicrobial activity: Antimicrobial Sensitivity Testing using Plant Extracts or Antibiotics

2. Concept 2 : Bio fertilizers: : inoculation of Tichoderma/Rhizobium/Azotobacter/VAM on seeds

3. Concept 3: Fermentation of Local Fruits for Alcohol or Acid Production

4. Concept 4: Estimation of pigments (chlorophyll, anthocyanin, lycopene) from fruits and vegetables

5. Concept 5 : Biomimetic Demonstrations: lotus leaf effect (water droplet rolling), self-cleaning surface

models, seashell hardness tests.

6. Concept 6 : Antagonist properties of Soil fungus using dual culture

Suggested Learning Resources:

Text books:

1. Bhushan, B. Biomimetics: Bioinspired Materials, Structures and Functions, Springer, pp. 1–300, 2017.

2. Singh, B.D. Biotechnology: Expanding Horizons, Kalyani Publishers, pp. 1–796, 2016.

3. Rastogi, S.C. Cell Biology, New Age International, pp. 1–400, 2013.

Reference books / Manuals:

1. Karp, G. Cell and Molecular Biology: Concepts and Experiments, Wiley, pp. 1–848, 2018.

2. Brown, T.A. Gene Cloning and DNA Analysis: An Introduction, Wiley-Blackwell, pp. 1–312, 2016.

3. Pelczar, M.J., Chan, E.C.S., Krieg, N.R. Microbiology: Concepts and Applications, Tata McGraw-Hill, pp. 1–896, 2009.

4. Voet, D., Voet, J.G., Pratt, C.W. Fundamentals of Biochemistry: Life at the Molecular Level, Wiley, pp. 1–1200, 2016.